Method of covalently bonding proteins to hydrophilic surfaces

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 17/08 (2006.01) A61L 33/00 (2006.01) C07K 17/10 (2006.01) C12N 11/08 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2072747

2072747 9109877 PCTABS00006 The invention discloses a method of covalently bonding protein to a surface coated with a nonionic hydrophilic polymer having a cloud point which is at least 5 ·C above the temperature of use of the final product. Immobilisation of the protein is carried at a temperature above 5·C below the cloud point of the nonionic hydrophilic polymer in the reaction medium.

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