G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) B01L 3/02 (2006.01) B01L 3/14 (2006.01) G01N 33/53 (2006.01) G01N 33/535 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2392681
The invention provides a microvolume assay method of an affinity reaction using a membrane of a small area in a microvolume reaction container for performing said affinity reaction between a target substance in a sample and a receptor capable of binding to the target substance, which method comprises the steps of: (1) taking a microvolume of the sample and loading to the membrane to allow the target substance in the sample to attach to the membrane; (2) placing the membrane into the microvolume reaction container, adding a solution containing an antibody (receptor) capable of binding to the target substance, wherein the volume of the solution is sufficient to soak the entire area of the membrane attaching the sample; (3) incubating the membrane in the reaction container for a time sufficient to allow the target substance attached to the membrane to bind to the receptor by an affinity reaction to form a target substance-receptor complex; and (4) detecting the target substance-receptor complex. A device for performing the microvolume assay method of the invention is also provided.
Chen Hsin-Huang
Robic
LandOfFree
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