Lateral flow and flow-through bioassay devices based on...

G - Physics – 01 – N

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G01N 33/558 (2006.01)

Patent

CA 2667702

Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.

L'invention concerne, dans des modes de réalisation, des dispositifs de dosage biologique à écoulement latéral et écoulement traversant basés sur des supports poreux à motif, leurs procédés de fabrication et leurs procédés d'utilisation. Selon un aspect de l'invention, un dispositif de dosage comprend : un support poreux et hydrophile; une barrière imperméable aux fluides comprenant un photorésist polymérisé, la barrière imprégnant sensiblement toute l'épaisseur du support poreux et hydrophile et définissant la limite d'une zone de dosage dans le support poreux et hydrophile; et un réactif de dosage dans la zone de dosage.

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