Methods and compositions for monitoring primer extension and...

Details Methods and compositions for monitoring primer extension and... Methods and compositions for monitoring primer extension and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2490530

The methods of invention include the use of control primers to monitor the efficacy of amplification and/or primer extension reactions, and possible subsequent use of these control products as sizing markers. The methods of the invention are applicable to single reactions as well as to high-throughput and multiplex systems, including array-based technologies. One embodiment of the invention comprises monitoring the efficacy of a reaction for the detection of polymorphisms in the scrapie gene.

Procédés consistant à utiliser des amorces de contrôle afin de contrôler l'efficacité de réactions d'amplification et/ou d'extension d'amorce et à mettre éventuellement en application ultérieurement ces produits de contrôle en tant que marqueurs d'évaluation. Ces procédés peuvent s'appliquer à des réactions simples ou à des systèmes très productifs et répétitifs, y compris des technologies basées sur des jeux ordonnés d'échantillons. Un mode de réalisation de l'invention consiste à contrôler l'efficacité d'une réaction visant à détecter des polymorphismes dans le gène de l'encéphalopathie spongiforme.

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