Microbial glyphosate resistant...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2570830

The present invention is based, in part, on a method for the identification of glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthase polypeptides and the isolation of the DNA molecules that encode the polypeptides. Also, chimeric DNA constructs are described that are useful to transform and express the glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthase polypeptide in bacteria and plant cells. The invention provides chimeric DNA molecules that are useful to transform plant cells, and the transformed plants, progeny, and parts thereof regenerated from the transformed plant cells.

La présente invention repose, en partie, sur un procédé pour l'identification de polypeptides de 5-enolpyruvyl-3-phosphoshikimate synthase résistants au glyphosate et l'isolation des molécules d'ADN codant pour les polypeptides. L'invention a également trait à des constructions d'ADN chimériques utiles pour la transformation et l'expression du polypeptide de 5-enolpyruvyl-3-phosphoshikimate synthase résistant au glyphosate dans des bactéries et des cellules végétales. L'invention a trait en outre à des molécules d'ADN chimériques utiles pour la transformation de cellules végétales, et à des plantes, leur descendance, et des parties de celles-ci régénérées à partir de cellules végétales transformées.

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