C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01) G01N 33/53 (2006.01) G01N 33/554 (2006.01) G01N 33/58 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01)
Patent
CA 2682472
The invention provides a genetic screening method for identifying a transfected cell expressing the polypeptide of interest. The methods allows for high throughput screening of recombinant cells for elevated levels of expression of the polypeptide of interest using methylcellulose comprising fluorescent protein A or G to improve detection and cloning. The invention also provides capture media, formulations and methods of making and using thereof.
L'invention propose un procédé de criblage génétique pour identifier une cellule transfectée exprimant le polypeptide d'intérêt. Le procédé permet le criblage à haut débit de cellules recombinantes pour des niveaux élevés d'expression du polypeptide d'intérêt en utilisant de la méthylcellulose comportant une protéine fluorescente A ou G pour améliorer la détection et le clonage. L'invention propose également des milieux de capture, des formulations et des procédés de fabrication et d'utilisation de ceux-ci.
Appelbaum Edward
Corisdeo Susanne
Ganguly Subiney
Kraichely Dennis M.
Mehta Sunil
Centocor Ortho Biotech Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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