Method for demonstration of a molecular event in a cell by...

G - Physics – 01 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

G01N 33/573 (2006.01) C12Q 1/37 (2006.01) G01N 33/50 (2006.01) G01N 33/542 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2531225

The invention relates to a method for demonstration of the occurrence of a molecular event, particularly in a cell, characterised by the detection of the "solubilisation" of a fixed protein marker (or the fixing of a solubilised protein marker) which is a direct or indirect marker for the occurrence of the particular molecular event. Said protein marker is present in the cell before the above detection, the cell being subjected to a permeabilisation of the plasma membrane before detection, which liberates the solubilised protein into the extracellular medium, the presence of the marker protein thus being detected in the cell or the extra-cellular medium by any appropriate means, which permits the detection of whether the solubilisation, or the fixing have taken place and, hence, the corresponding molecular event.

La présente invention concerne un procédé de mise en évidence de la survenue d'un événement moléculaire particulier dans une cellule, caractérisé en ce que on détecte la "solubilisation" d'une protéine marqueur "fixée" (respectivement la "fixation" d'une protéine marqueur "solubilisée") qui est un marqueur direct ou indirect de la survenue de l'évènement moléculaire particulier, laquelle protéine marqueur est présente dans la cellule avant la détection précédente, la cellule étant, avant la détection, soumise à une perméabilisation de la membrane plasmique qui libère la protéine solubilisée dans le milieu extracellulaire, la présence de la protéine marqueur étant alors détectée dans la cellule ou le milieu extracellulaire par tout moyen approprié, ce qui permet de déterminer si la solubilisation, respectivement la fixation, ont eu lieu et donc l'événement moléculaire correspondant.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for demonstration of a molecular event in a cell by... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for demonstration of a molecular event in a cell by..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for demonstration of a molecular event in a cell by... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1901563

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.