Transgenic plants producing a pap ii protein

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 5/14 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2329150

Disclosed are recombinant plant cells, plant cell parts, plant parts and transgenic plants containing a DNA molecule comprising a sequence encoding a Pokeweed Antiviral Protein (PAP) II protein. PAP II proteins include full length, wild-type PAP II and substantially nontoxic mutants or analogs including fragments thereof truncated at the C-terminus and other PAP II proteins having an intact catalytic active site amino acid residue E172 but that also have at least one amino acid substitution or deletion, and possess anti-viral and/or anti-fungal activity. DNA molecules comprising sequences encoding the mutants or analogs, as well as the isolated and purified PAP II proteins per se, are also disclosed. Methods of identifying nontoxic PAP II mutants are further disclosed. Transgenic plants that produce a PAP II protein exhibit anti-viral and/or anti-fungal activity. Virtually all flowering plants are included. Seed derived from the transgenic plants are also provided.

L'invention concerne des cellules de plantes recombinées, des parties de cellules de plantes, des parties de plantes et des plantes transgéniques, contenant une molécule d'ADN comprenant une séquence codant une protéine appelée protéine antivirale de type II de l'épinard du Canada (PAP). Les protéines PAP II contiennent la PAP II du type sauvage et pleine longueur et des mutants ou des analogues sensiblement non toxiques, comprenant des fragments desdites protéines, tronqués au niveau de C-terminal et d'autres protéines PAP II possédant un reste d'acide aminé E172, à site catalytique actif, intact, mais également au moins une substitution ou une délétion aminoacide, et présentent une activité antivirale et/ou antifongique. Les molécules d'ADN comprenant les séquences codant pour les mutants ou analogues, ainsi que les protéines PAP II isolées et purifiées, connues en soi, sont décrites, ainsi que des procédés d'identification de mutants de PAP II non toxiques. Les plantes transgéniques qui produisent une protéine PAP II présentent une activité antivirale et/ou antifongique. Pratiquement toutes les plantes à fleur sont incluses. Les semences dérivées des plantes transgéniques sont décrites.

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