Hemoglobin mutants with increased soluble expression and/or...

Details Hemoglobin mutants with increased soluble expression and/or... Hemoglobin mutants with increased soluble expression and/or...

C - Chemistry, Metallurgy

07

K

C07K 14/805 (2006.01) A61K 38/42 (2006.01)

Patent

CA 2618690

A hemoglobin mutant with reduced rate of reaction with nitric oxide selected from the following mutation in the alpha subunit: E11(Val-- > Leu) and E7(His-- > Gln); E11(Val-- > Phe or Trp) and E7(His-- > Gln); E11(Val-- > Phe or Trp or Leu) and E7(His--- > Gln) and G8(Leu-- > Phe or Trp); B10(Leu-- > Phe) and E4(Val-- > Leu); B10(Leu-- > Trp) and E4(Val-- > Leu); B10(Leu-- > Trp) and E7(His-- > Gln); B10(Leu-- > Trp) and E11(Val--- > Phe); B10(Leu-- > Trp) and E11(Val-- > Trp); B10(Leu-- > Trp) and E11(Val-- > Leu) and G8(Leu-- > Trp); B10(Leu-- > Trp) and E11(Val-- > Leu) and G8(Leu-- > Phe); B10(Leu--- > Trp) and E11(Val-- > Phe) and G8(Leu-- > Trp); B10(Leu-- > Trp) and E11(Val-- > Phe) and G8(Leu-- > Ilc); B10(Leu-- > Trp) and E7(His-- > Gln) and E11(Val-- > Leu) and G8(Leu-- > Trp); B10(Leu-- > Trp) and E11(Val-- > Trp) and G8(Leu-- > Trp); E11(Val--- > Leu) and G8(Leu-- > Phe); E11(Val-- > Leu) and G8(Leu-- > Trp); B13(Met-- > Phe or Trp); G12(Leu-- > Phe or Trp); or B14(Phe-- > Trp).

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