Compositions and methods for use in analytical reactions

Details Compositions and methods for use in analytical reactions Compositions and methods for use in analytical reactions

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2715385

Compositions, methods, substrates and systems for use in analysis of single molecule reactions and particularly single molecule nucleic acid sequence analysis. Compositions that include non- reactive, distinguishable or undetectable competi-tive substrates for the reaction system of interest are provided, as well as their use in systems and substrates for such applications, such compounds typically preferably polyphosphate chains or analogous structures.

La présente invention concerne des compositions, procédés, substrats et systèmes utilisables dans le cadre de l'analyse de réactions impliquant une unique molécule et, en particulier, dans le cadre de l'analyse de la séquence d'une unique molécule d'acide nucléique. L'invention concerne donc des compositions comprenant des substrats compétitifs non réactifs, perceptibles ou indétectables pour le système réactionnel d'intérêt, ainsi que leur utilisation dans des systèmes et des substrats pour lesdites applications, lesdits composés correspondant, typiquement et de préférence, à des chaînes de polyphosphate ou à des structures analogues.

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