Protein expression yield enhancement in cell-free protein...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 5/00 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2560504

Compositions and methods are provided for the in vitro synthesis of biological molecules in reaction mixtures comprising anti-foam agents. The reaction mix comprising antifoam agent may be a scaled up reaction, e.g. in reaction volume greater than at least about 15 ul. Reactions may be performed in various reactors, as known in the art, which include stirred reactors, bubble-column reactors; and the like.

La présente invention se rapporte à des compositions et à des procédés permettant la synthèse <i>in vitro</i> de molécules biologiques dans des mélanges réactionnels comportant des agents anti-mousse. Le mélange réactionnel comprenant un agent anti-mousse peut être produit par une réaction avec augmentation de volume, par exemple avec un volume réactionnel supérieur à au moins 15 µl environ. Les réactions peuvent être mises en oeuvre dans divers réacteurs, tels que ceux utilisés dans l'art antérieur, et notamment dans des réacteurs à agitation, des réacteurs à colonnes à bulles et analogues.

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