Specialized dual condensation/epimerization domain in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/52 (2006.01) C07K 1/04 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/90 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2430580

The present invention relates to domains of non-ribosomal peptide synthetases (NRPSs) that exhibit dual condensation and epimerization activities. The "dual condensation/epimerization NRPS domains" of them present invention allow for the incorporation of non-proteinogenic substrates; such as D-amino acids, into peptide products. These dual condensation/epimerization NRPS domains may further be used to modify the stereochemistry of synthesized peptides at selected amino acid sites.

L'invention porte sur des domaines de synthétases de peptides non ribosomiques (NRPS) qui présentent des activités de double condensation et épimérisation. Ces </= domaines de double condensation/epimérisation NRPS >/= permettent l'introduction de substrats non protéinogéniques, tels que les acides aminés D, dans des produits peptidiques. Ces domaines de double condensation/epimérisation NRPS peuvent également servir à modifier la stéréochimie de peptides synthétisés au niveau des sites d'acides aminés sélectionnés.

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