C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) C07K 14/415 (2006.01)
Patent
CA 2108897
The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome expression and a microorganism transformed therewith. PIP genome of the present invention has nucleotide homology of about 82%, compared with the genome of Phytolacca americana antiviral protein isolated from Phytolacca americana L., which is closely related to the Phytolacca insularis Nakai. PIP cDNA is consist of 918bp of one open reading frame and termination codon; and polyadenylation signal which is ubiquitous in mRNA of most plants and animals, appears to be located in the upstream of 33bp from the polyadenylation site. Recombinant PIP of the invention was proved to inhibit the growth of E. coli HB101 transformed with said expression vector.
Les inventeurs ont découvert un nouveau génome codant la protéine antivirale de Phytolacca insularis (PPI) isolée de Phytolacca insularis Nakai, et mis au point un vecteur recombinant pour l'expression du génome de ladite PPI ainsi qu'un microorganisme transformé avec ce vecteur. Le génome de la PPI contient environ 82 % des mêmes nucléotides que la protéine antivirale de Phytolacca americana isolée de Phytolacca Americana L., qui est étroitement apparenté à Phytolacca insularis Nakai. L'ADNc de la PPI comprend un cadre de lecture ouvert et un codon de terminaison de 918 pb; et le signal de polyadénylation, qui est ubiquiste dans l'ARNm de la plupart des plantes et animaux, semble être situé en amont de 33 pb du site de polyadénylation. La PPI recombinante inhibe la croissance de E. coli HB101 transformé avec ledit vecteur d'expression.
Choi Kyu-Whan
Jeon Hong-Seob
Kim Man-Keun
Lee Kwan-Ho
Moon Young-Ho
Cassan Maclean
Viromedica Pacific Limited
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