Methods, compositions and kits for isothermal amplification...

Details Methods, compositions and kits for isothermal amplification... Methods, compositions and kits for isothermal amplification...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2621146

Methods and compositions are described for isothermal nucleic acid amplification of a nucleic acid template strand by using an oligonucleotide primer that includes an AT-rich nucleotide sequence and a polymerase having strand displacement activity.

L'invention concerne des procédés et des compositions servant à l'amplification isotherme d'acides nucléiques. L'amplification isotherme d'un brin complémentaire d'acide nucléique se fait au moyen d'une amorce oligonucléotidique contenant une séquence nucléotidique riche en AT, ainsi qu'au moyen d'une polymérase ayant une activité de déplacement de brin.

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