Glycerol-3-phosphate dehydrogenase gene and use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/04 (2006.01)

Patent

CA 2638789

The present invention relates to a gene encoding glycerol-3-phosphate dehydrogenase and use thereof, in particular, a brewer's yeast which produces alcoholic beverages with superior body and mellowness, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast whose ability of producing glycerol, which contribute to body and mellowness of products, is enhanced by amplifying expression level of GPD1 or GPD2 gene encoding a Gpd1p or Gpd2p which is a glycerol-3-phosphate dehydrogenase in brewer's yeast, especially non-ScGPD1 gene or non-ScGPD2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.

La présente invention porte sur un gène codant glycérol-3-phosphate déshydrogénase et sur son utilisation, notamment, sur une levure de bière qui produit des boissons alcoolisées présentant plus de consistance et de friabilité, sur des boissons alcoolisées produites à partir de cette levure et sur un procédé de production des boissons précitées. L'invention porte plus particulièrement sur une levure dont la capacité à produire le glycérol, lequel confère aux produits consistance et friabilité, est améliorée par l'amplification du niveau d'expression du gène GPD1 ou GPD2 codant une Gpd1p ou Gpd2p qui est une glycérol-3-phosphate déshydrogénase de la levure de bière, notamment un gène non-ScGPD1 ou un gène non-ScGPD2 spécifique d'une levure de bière de fermentation basse. L'invention porte également sur un procédé de production de boissons alcoolisées utilisant cette levure, etc.

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