C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/66 (2006.01) C12N 15/70 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01)
Patent
CA 2373690
Methods are provided for producing an expression vector. In the subject methods, donor and acceptor vectors are combined in the presence of a recombinase to produce an expression vector that includes a first and second recombinase recognition site oriented in the same direction, wherein the first and second recombination sites are able to recombine with each other. In the subject methods, one of the donor and acceptor vectors includes a single recombinase recognition site while the other includes two recombinase recognition sites. Also provided are compositions for use in practicing the subject methods, including the donor and acceptor vectors themselves, as well as systems and kits that include the same. The subject invention finds use in a variety of different applications, including the transfer or cloning of a nucleic acid of interest from a first vector into one or more expression vectors, etc.
Cette invention concerne des procédés permettant de produire un vecteur d'expression. Selon ces procédés, on fait se combiner des vecteurs donneurs et receveurs en présence d'une recombinase afin d'obtenir un vecteur d'expression qui comprend un premier et un second site de reconnaissance de la recombinase orientés dans la même direction. Les premier et second sites de recombinaison sont capables de se recombiner mutuellement. Toujours selon ces procédés, l'un des vecteurs donneur ou receveur comprend un seul site de reconnaissance de la recombinase alors que l'autre vecteur en comprend deux. L'invention concerne également des compositions servant à appliquer lesdits procédés, dont les vecteurs donneurs et receveurs eux-mêmes ainsi que des systèmes et des kits correspondants. Cette invention convient pour diverses applications, telles que le transfert ou le clonage d'acides nucléiques d'intérêt à partir d'un premier vecteur en un ou plusieurs vecteurs d'expression.
Clontech Laboratories Inc.
Fetherstonhaugh & Co.
LandOfFree
Recombinase-based methods for producing expression vectors... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinase-based methods for producing expression vectors..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinase-based methods for producing expression vectors... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1918849