G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 21/76 (2006.01) G01N 21/64 (2006.01)
Patent
CA 2648382
Disclosed are methods, kits, and compositions for fluorescent detection of single biomolecules, e.g. proteins, using highly sensitive fluorescent dyes and a flow cell with one interrogation space for irradiation by focused laser beam. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.
La présente invention concerne des procédés, des trousses et des compositions permettant la détection extrêmement sensible de molécules. Les procédés, les trousses et les compositions se révèlent utiles pour déterminer des concentrations de molécules dans des échantillons à des niveaux de 1 femtomolaire, 1 attomolaire, ou moins. Les procédés, les trousses et les compositions permettent également la détermination de concentration couvrant une large plage, notamment de 7-log, sans avoir à recourir à une dilution de l'échantillon.
Goix Philippe
Held Douglas
Livingston Richard
Puskas Robert
Todd John
Borden Ladner Gervais Llp
Singulex Inc.
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