Chimeric primers for improved nucleic acid amplification...

Details Chimeric primers for improved nucleic acid amplification... Chimeric primers for improved nucleic acid amplification...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2691942

Methods are provided for amplification of a nucleic acid sequence. The method use RNA/DNA chimeric oligonucleotides as primers. The primers have RNA residues scattered along their length and no two ribonucleotides in the prime are adjacent to one another. The methods are useful for reducing non-specific amplification products, such as primer dimers. The invention also provides kits comprising RNA/DNA chimeric oligonucleotide primers for practicing the amplification methods.

L'invention concerne des procédés pour l'amplification d'une séquence d'acides nucléiques. Les procédés utilisent des oligonucléotides chimères d'ARN/ADN en tant qu'amorces. Les procédés sont utiles pour réduire la quantité de produits d'amplification non spécifiques tels que des dimères d'amorce. L'invention concerne également des kits comprenant des amorces oligonucléotides chimères d'ARN/ADN pour pratiquer les procédés d'amplification.

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