Method of mycotoxin detection

C - Chemistry – Metallurgy – 07 – H

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C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2715425

The presence of mycotoxins in agricultural products necessitates large scale testing of a wide range of sample material to ensure the safety of food and feed. The mycotoxin ochratoxin A represents an enablement for all mycotoxins as the level of sensitivity necessary for regulatory requirements for this compound at the part per billion level are as low or lower than any other mycotoxin. This invention describes the identification of a set of DNA ligands with sufficiently high binding affinity and specificity for ochratoxin A to enable an improvement over existing methods for the separation, concentration and quantitative determination of ochratoxin A in sample material.

La présence de mycotoxines dans les produits agricoles requiert des essais à grande échelle, sur un large éventail de matériaux échantillons, pour garantir l'innocuité des produits destinés à l'alimentation humaine et animale. La mycotoxine ochratoxine A est représentative de toutes les mycotoxines car le niveau de sensibilité nécessaire pour les prescriptions réglementaires de ce composé, en parties par milliard, est égal ou inférieur à celui de toutes les autres mycotoxines. Cette invention concerne l'identification d'un jeu de ligands ADN dotés d'une affinité de liaison et d'une spécificité suffisamment élevées à l'ochratoxine A pour procurer une amélioration par rapport aux procédés existants pour la séparation, la concentration et la détermination quantitative de l'ochratoxine A dans le matériau échantillon.

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