C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/12 (2006.01) C07K 1/22 (2006.01) C07K 14/33 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2191774
A method is provided for the purification of Clostridium difficile Toxin A comprising reacting impure Toxin A with immobilized mono-specific polyclonal antibodies. The polyclonal antibodies are coupled to a hydrazide group containing matrix such as hydrazide activated agarose gel. The immobilized antibody is specific for Toxin A and will greatly purify Toxin A from a Toxin A containing solution. Antibodies raised to Toxin A purified according to the method are of higher activity than antibodies produced from prior art purified Toxin A.
On décrit un procédé de purification de la toxine A de Clostridium difficile consistant à faire réagir une toxine A impure avec des anticorps polyclonaux monospécifiques immobilisés. Ces anticorps polyclonaux sont couplés à un groupe hydrazide contenant une matrice telle que du gel d'agarose activé par hydrazide. L'anticorps immobilisé est spécifique de la toxine A et il va purifier de façon importante la toxine A provenant d'une solution contenant celle-ci. Des anticorps dirigés contre la toxine A purifiée selon ce procédé possèdent une activité supérieure à celle d'anticorps produits à partir de toxine A purifiée à l'aide de techniques actuelles.
Gowling Lafleur Henderson Llp
Lee Laboratories Inc.
LandOfFree
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