C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/34 (2006.01) C12Q 1/37 (2006.01)
Patent
CA 2651917
The present invention provides a method for the detection of an erizyme E1 in a liquid sample comprising the steps of: a) providing a complex (Sa-Sb-M), wherein (Sa-Sb) is a substrate S of E1 cleavable into Sa and Sb by E1, and M is a marker linked to Sb, b) incubating the sample with the complex under conditions enabling the cleavage of S into Sa and Sb by E1, c) separating non-cleaved complex (Sa-Sb-M) from the sample, and d) measuring M in the sample. Furthermore, the present invention further provides kits and devices for the detection of an enzyme E1.
La présente invention concerne un procédé pour la détection d'une enzyme E1 dans un échantillon liquide comprenant les étapes consistant à : a) produire un complexe (Sa-Sb-M), dans lequel (Sa-Sb) est un substrat S de E1 clivable en Sa et Sb par E1, et M est un marqueur lié à Sb, b) incuber l'échantillon avec le complexe dans des conditions permettant le clivage de S en Sa et Sb par E1, c) séparer le complexe non clivé (Sa-Sb-M) de l'échantillon, et d) mesurer M dans l'échantillon. De plus, la présente invention concerne en outre des kits et des dispositifs pour la détection d'une enzyme E1.
Ambar Abraham
Ambar Benjamin Badri
Bcf Llp
Bio Pur Ag
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