C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) B01J 21/10 (2006.01) B01J 23/40 (2006.01) C01B 3/38 (2006.01) C01B 3/40 (2006.01) C12N 11/00 (2006.01) C12N 11/16 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2481986
The present invention relates to a method of transfecting cells comprising applying cells directly onto nucleic acids which are immobilized in transfection complexes on a surface and which transfect the cells. Preferably, the nucleic acids are immobilized in an array. In another aspect of the present invention, the method further includes expression of the nucleic acids in the transfected cells. In yet another aspect of the present invention, the method further comprises detecting the expression of the nucleic acids in the transfected cells.
La présente invention concerne un procédé de transfection de cellules consistant à appliquer des cellules directement sur des acides nucléiques, lesquels sont immobilisés dans des complexes de transfection sur une surface et lesquels transfectent les cellules. De préférence, les acides nucléiques sont immobilisés en un ensemble. Dans un autre aspect de la présente invention, le procédé comprend également l'expression des acides nucléiques dans les cellules transfectées. Dans encore un autre aspect de la présente invention, le procédé comprend aussi la détection de l'expression des acides nucléiques dans les cellules transfectées.
Smart & Biggar
The Regents Of The University Of Michigan
LandOfFree
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