C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2702584
The present invention provides compositions, methods and kits for use in the detection of small RNA sequences, which allow for rapid and robust amplification and detection. The methods provide improved sensitivity and efficiency in the amplification-based detection of small RNA sequences by incorporating one or more base-modified duplex-stabilizing dNTPs during reverse transcription and/or amplification.
La présente invention concerne des compositions, des procédés et des kits destinés à être utilisés dans la détection de petites séquences d'ARN, qui permettent une amplification et une détection rapides et puissantes. Lesdits procédés apportent une sensibilité et une efficacité accrues dans la détection basée sur l'amplification de petites séquences d'ARN, par l'incorporation d'un ou de plusieurs dNTP à stabilisation de double hélice à base modifiée lors de la transcription et/ou de l'amplification inverse.
Borden Ladner Gervais Llp
Kutyavin Igor Vassily
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