System and method for determining the presence of methylated...

Details System and method for determining the presence of methylated... System and method for determining the presence of methylated...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2425413

The present invention relates to a method for determining the presence of a methylated cytosine in a first sample comprising a first nucleotide containing compound. The first sample and a reference NCC are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first NCC and the reference NCC is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference NCCs. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference NCCs.

La présente invention concerne un procédé qui permet de déterminer la présence d'une cytosine méthylée dans un premier échantillon comprenant un composé contenant un premier nucléotide. Le premier échantillon et un composé contenant un nucléotide (CCN) de référence sont soumis à une électrophorèse en présence d'au moins un colorant intercalant. Pendant l'électrophorèse, la température du premier CCN et du CCN de référence est modifiée de manière suffisante pour changer la mobilité électrophorétique d'au moins un des deux CCN. Des données d'intensité de la fluorescence sont obtenues, ces données indiquant la présence du premier CCN et du CCN de référence.

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