Method for producing .beta.-glucanase and xylanase, and...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/24 (2006.01) C12N 1/00 (2006.01) C12P 19/00 (2006.01)

Patent

CA 2747201

A problem to be solved is to produce cellulase having excellent ability to decompose a cellulosic resource containing xylan at low cost. A method for producing .beta.-glucanase and xylanase comprising the step of culturing a microorganism classified under the genus Trichoderma by using a liquid culture medium which contains (a) a pulp derived from paper which has not been subjected to heat treatment nor alkali treatment as a carbon source and (b) an ammonia nitrogen or an amino nitrogen as a nitrogen source.

L'objectif de l'invention est de produire une cellulase ayant une excellente aptitude à la décomposition d'une ressource cellulosique contenant du xylane à faible coût. L'invention porte sur un procédé de production de ß-glucanase et de xylanase, qui comprend l'étape consistant à cultiver un microorganisme appartenant au genre Trichoderma à l'aide d'un milieu de culture liquide contenant (a) une pâte issue du papier qui n'a pas été chauffée ni traitée par un alcali en tant que source de carbone et (b) un azote ammoniacal ou un azote d'amino en tant que source d'azote.

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