Method for screening highly active proteases and inhibitors

Details Method for screening highly active proteases and inhibitors Method for screening highly active proteases and inhibitors

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 1/04 (2006.01) C07K 14/81 (2006.01) C12N 9/64 (2006.01) C12N 15/62 (2006.01) C12Q 1/02 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2419860

A method of screening a protease gene library for a gene encoding a protease of interest, the method comprising the steps of introducing a first gene of the protease gene library and a second gene encoding a protease inhibitor into a host cell; cultivating the host cell, wherein the cell expresses the first and the second genes to produce a complex of a protease and the inhibitor; dissociating the inhibitor from the complex; and selecting the protease of interest and isolating the encoding gene.

Procédé pour cribler une bibliothèque génétique des protéases à la recherche d'un gène codant pour une protéase d'intérêt, le procédé consistant à introduire un premier gène de la bibliothèque génétique des protéases et un deuxième gène codant un inhibiteur de protéase dans une cellule hôte; cultiver la cellule hôte, la cellule exprimant les premier et deuxième gènes pour produire un complexe d'une protéase et de l'inhibiteur; dissocier l'inhibiteur du complexe; et sélectionner la protéase d'intérêt puis isoler le gène codant.

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