Probe for protein-protein interaction analysis, and method...

G - Physics – 01 – N

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G01N 33/68 (2006.01) C12N 15/09 (2006.01) G01N 33/531 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2385830

A probe for protein-protein interaction analysis applicable to various proteins which enables simple analysis in high accuracy, and a method for analyzing interaction of two proteins is provided. Protein splicing from the probe is caused by protein-protein interaction, and a physiochemically or biochemically detectable protein is regenerated.

Cette invention a trait à une sonde permettant d'analyser une interaction protéine/protéine et notamment, à une sonde permettant d'analyser des interactions entre protéines, quelles qu'elles soient, commodément et de la façon la plus précise qui soit. L'invention porte également sur une méthode d'analyse d'une interaction protéine/protéine utilisant cette sonde. On utilise, dans le cadre de cette méthode, une sonde pour analyser une interaction entre deux protéines, laquelle interaction déclenche un épissage de protéines et une protéine pouvant être détectée par voie physico-chimique ou biochimique est régénérée.

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