Igm production by transformed cells and methods for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) A61K 39/395 (2006.01) C07K 16/18 (2006.01) C12N 5/10 (2006.01) C12P 21/02 (2006.01) G01N 27/447 (2006.01) G01N 33/53 (2006.01) G01N 33/561 (2006.01)

Patent

CA 2532966

Pentamer IgM can be obtained by locating genes respectively encoding H chain, L chain and J chain on a single vector and then transforming into an appropriate host cell. The gene encoding J chain may be transferred via cotransformation. In the case where the J chain is not expressed, the product is obtained as hexamer IgM. The transformant thus obtained shows a high IgM productivity. It is also intended to provide a method whereby a polymer IgM can be separated and quantified.

On peut obtenir de l'IgM pentamère par la localisation de gènes codant respectivement la chaîne H, la chaîne L et la chaîne J sur un seul vecteur suivie par la transformation en une cellule hôte appropriée. Le gène codant la chaîne J peut être transféré par co-transformation. Dans le cas où la chaîne J n'est pas exprimée, le produit obtenu est un IgM hexamère. Le transformant ainsi obtenu présente une productivité IgM élevée. Cette invention se rapporte également à un procédé de séparation et de quantification d'un IgM polymère.

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