Process and reagent for determining triglycerides

Details Process and reagent for determining triglycerides Process and reagent for determining triglycerides

C - Chemistry, Metallurgy

12

Q

150/3.3, 150/15.

C12Q 1/34 (2006.01) C12Q 1/44 (2006.01) C12Q 1/48 (2006.01) C12Q 1/61 (2006.01)

Patent

CA 1148456

ABSTRACT OF THE DISCLOSURE For the determination of triglycerides by ester splitting with lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with ATP in the presence of glycerokinase with the formation of glycerol-1-phospate and ADP and determination of one of these formed substances, a glycero- kinase from Bacillus stearothermophilis is combined with an activator selected from detergents, phenol derivatives and aniline derivatives; in addition, a suitable reagent contains lipase, ATP and glycerokinase from Bacillus stearothermophilis in combination with said activator and a system for determining glycerol-1-phospate or ADP, and optionally esterase; the activator is suitably a detergent, especially a polyethylene oxide ester, a polyethylene oxide ether, a bile acid or an alkyl sulphate.

365792

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