Process for glucan preparation and therapeutic uses of glucan

Details Process for glucan preparation and therapeutic uses of glucan Process for glucan preparation and therapeutic uses of glucan

C - Chemistry, Metallurgy

08

B

C08B 37/00 (2006.01) A61K 31/715 (2006.01) A61K 31/716 (2006.01) A61L 26/00 (2006.01) A61L 27/00 (2006.01) C12P 19/04 (2006.01)

Patent

CA 2214899

A process for the production of .beta.-(1,3)(1,6) glucan from a glucan containing cellular source is described, together with compositions and uses/methods of treatment involving glucan. The process of the invention comprises the steps of: (a) extracting glucan containing cells with alkali and heat, in order to remove alkali soluble components; (b) acid extracting the cells of step (a) with an acid and heat to form a suspension; (c) extracting the suspension obtained of step (b) or recovered hydrolyzed cells with an organic solvent which is non-miscible with water and which has a density greater than that of water separating the resultant aqueous phase, solvent containing phase and interface so that substantially only the aqueous phase comprising .beta.-(1,3)(1,6) glucan particulate material remains; wherein the extraction with said organic solvent provides separation of glucan subgroups comprising branched .beta.-(1,3)(1,6)-glucan, and essentially unbranched .beta.-(1,3) glucan which is associated with residual non-glucan contaminents; and (d) drying the glucan material from step (c) to give microparticulate glucan.

L'invention a pour objet un procédé de production de beta -(1,3)(1,6) glucane à partir d'une source cellulaire contenant du glucane. L'invention traite également de compositions et des applications/méthodes de traitement mettant en oeuvre le glucane. Le procédé selon l'invention comprend les étapes consistant: (a) à extraire les cellules contenant du glucane à l'aide d'alcali et en les chauffant, afin de retirer les compsants solubles à l'alcali; (b) à extraire à l'acide les cellules de l'étape (a) à l'aide d'un acide et en les chauffant pour former une suspension; (c) à extraire la suspension obtenue à l'étape (b); ou les cellules hydrolysées extraites, à l'aide d'un solvant organique non miscible à l'eau et dont la densité est supérieure à celle de l'eau, et séparer la phase aqueuse obtenue, la phase contenant le solvant et l'interface de sorte que l'on obtient uniquement la phase aqueuse comprenant la matière particulaire de beta -(1,3)(1,6) glucane. Selon ce procédé, l'extraction à l'aide du solvent organique assure la séparation des sous-groupes de glucane comprenant du beta -(1,3)(1,6) glucane ramifié, et essentiellement du beta -(1,3) glucane non ramifié qui est associé aux contaminants résiduaires autre que le glucane. Le procédé comprend enfin l'étape (d) consistant à sécher la matière de glucane obtenue à l'étape (c) pour donner du glucane microparticulaire.

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