Enhanced gene expression system

G - Physics – 01 – N

Patent

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Details

G01N 33/567 (2006.01) C12Q 1/68 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2480454

The present disclosure provides an enhanced gene transcription system including a systematic method of selecting efficient promoter-enhancers, of optimizing plasmid design and increasing transcription of a cDNA of interest in transfected target cells. The present invention identifies abundantly, selectively expressed genes and creates plasmids comprising the promoters- enhancers of those genes.

L'invention concerne un système de transcription génique amélioré et une méthode systématique permettant de sélectionner des activateurs de promoteurs améliorés, d'optimiser la création de plasmides et d'augmenter la transcription d'un ADNc d'intérêt dans des cellules cibles transfectées. L'invention permet d'identifier des gènes exprimés sélectivement et en abondance et de créer des plasmides comprenant les activateurs de promoteurs de ces gènes.

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