Methods of increasing protein expression levels

Details Methods of increasing protein expression levels Methods of increasing protein expression levels

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C07K 1/107 (2006.01) C07K 14/715 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/28 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2466298

The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence, preferably the tumor necrosis factor receptor, is subtituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a casteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.

L'invention concerne des procédés d'augmentation des niveaux d'expression de protéines, dans lesquels au moins un aminoacide d'une séquence d'aminoacides de protéine est substitué par l'aminoacide proline. La substitution s'effectue, de préférence dans 15 aminoacides, idéalement 10 et encore mieux 5 aminoacides d'un reste d'aminoacide de cystéine. L'invention ne porte pas que sur des procédés pour des polypeptides à substitutions par la proline, mais également sur des polynucléotides à substitutions par des codons pour lesquels un codon pour un aminoacide, excepté la proline, est substitué par un codon codant pour la proline

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