Dna polymorphism identity determination using flow cytometry

Details Dna polymorphism identity determination using flow cytometry Dna polymorphism identity determination using flow cytometry

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2308599

Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.

Il est fait en sorte que des amorces conçues pour être immobilisées sur des microsphères puissent s'anneler à un brin d'ADN en cours d'examen, et qu'elles soient ou bien agrandies par un ADN polymérase à l'aide de didéoxynucléotides fluorescents ou bien liées par une ADN ligase à des oligonucléotides marqueurs fluorescents. La fluorescence du didéoxynucléotide ou de l'oligonucléotide marqueur attaché à l'amorce immobilisée se mesure par la cytométrie de flux, ce qui permet d'identifier le polymorphisme nucléotidique sur le brin d'ADN.

Affiliated with

Also associated with

Rating

Dna polymorphism identity determination using flow cytometry does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dna polymorphism identity determination using flow cytometry, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dna polymorphism identity determination using flow cytometry will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1945600