Microfluidic system with electrofluidic and electrothermal...

Details Microfluidic system with electrofluidic and electrothermal... Microfluidic system with electrofluidic and electrothermal...

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12M 1/36 (2006.01) C12M 1/38 (2006.01) C12M 1/40 (2006.01)

Patent

CA 2510539

A novel method for transporting and/or monitoring a fluid in a multi-port device 400, 800, 1000 used in a microfluidic system is provided. The method comprises providing amplification reaction reagents within at least a first portion of a first microscale channel, and repeatedly cycling a temperature within the at least first portion to temperatures appropriate for carrying out melting, annealing and extension reactions within the amplification reaction. The amplification reagents comprising template nucleic acid, a primer sequence, nucleoside triphosphates and polymerase enzyme. The cycling temperature comprises variably applying an electrical current through the first portion of the first channel to heat a fluid in the first portion of the first channel.

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