Method for cloning animals with targetted genetic...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A01K 67/00 (2006.01) A01K 67/027 (2006.01) C12N 15/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2396210

An improved method of nuclear transfer employing long-term cultured somatic cells as the donor cells and enucleated oocytes as the recipient cells to produce dividing cybrids. Such cybrids are useful for developing viable animals clones when nurtured in a suitable host environment.

L'invention concerne un procédé amélioré de transfert nucléaire utilisant des cellules somatiques obtenues par culture à long terme en tant que cellules donneuses et ovocytes énucléés en tant que cellules receveuses en vue de produire des cybrides de division. De tels cybrides sont utilisés pour le développement de clones animaux viables lorsqu'ils sont nourris dans un environnement hôte approprié.

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