Apparatus and methods for producing and using high-density...

Details Apparatus and methods for producing and using high-density... Apparatus and methods for producing and using high-density...

C - Chemistry, Metallurgy

12

M

C12M 1/00 (2006.01) C12N 5/07 (2010.01) C12M 1/04 (2006.01) C12M 1/12 (2006.01) C12M 3/06 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2360916

Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cells at high cell density with wild type and recombinant baculoviruses to produce baculovirus and DNA or gene or expession products. The apparatus comprises three interconnected modules, the cell culturing loop (100), the media replenishment loop (200) and the hollow fiber dialysis device (300). Cells from the bioreactor (110) are transported through the lumen space (310) of hollow fiber dialysis device and media from the media replenishment loop (200) is pumped through the extra-lumen space (320). Prior to re-entering the bioreactor the cells with media have oxygen added via the oxygenator (151).

L'invention porte sur un appareil et sur des proc~d~s de prolif~ration de cellules ~ une haute densit~, sur leurs produits et utilisations. L'invention porte ~galement sur l'utilisation de ce proc~d~ dans la prolif~ration de cellules d'insectes ~ haute densit~ telles que des cellules de Spodptera frugiperda Sf900+ ~ haute densit~ avec des baculovirus de g~notype sauvage et de recombinaison de fa×on ~ g~n~rer des baculovirus et des produits d'ADN, g~niques ou d'expression. Cet appareil comprend trois modules interconnect~s, la boucle de culture cellulaire (100), la boucle de r~approvisionnement en milieu de culture (200) et le dispositif de dialyse ~ fibre creuse (300). Les cellules provenant du bior~acteur (110) sont transport~es via l'espace intraluminal (310) du dispositif de dialyse ~ fibre creuse et le milieu provenant de la boucle de r~approvisionnement en milieu de culture (200) est pomp~ via l'espace extraluminal (320). Avant de les r~introduire dans le bior~acteur, on ajoute aux cellules et ~ leur milieu de culture de l'oxyg­ne fourni par l'oxyg~nateur (151).

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