Image based quantitation of molecular translocation

Details Image based quantitation of molecular translocation Image based quantitation of molecular translocation

G - Physics

01

N

G01N 33/50 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2598602

The use of a multi-spectral imaging system, cell compartment markers, and molecular probes in a method for measuring movement of molecules within a cell by correlation analysis is provided, including measuring molecular movement to a particular compartment in adherent and non-adherent cells, e.g. in response to biological stimuli. A compartment in the cell is defined by the image of a specific compartment marker, e.g., a nuclear fluorescent stain. Molecule location is provided by a probe labeled with a different fluorochrome. A mask is generated based on the compartmental marker, and a correlation measurement is made between the locations of the molecular probe and the compartment marker. The correlation value between the regions defined by the compartment mask and molecular probe gives a quantitative measurement of the translocation of the molecule. The use of only a single masking function simplifies measurement of molecular translocation within a cell.

L'invention concerne l'utilisation d'un système d'imagerie, de marqueurs de compartiment cellulaire et de marqueurs moléculaires dans des procédés visant à mettre en corrélation le déplacement des molécules, à l'intérieur d'une cellule, vers un compartiment particulier. Les procédés comportent les étapes consistant à mesurer et à mettre en corrélation le déplacement des molécules dans des cellules adhérentes et non adhérentes.

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