C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/078 (2010.01) C12N 5/0781 (2010.01) C07K 16/00 (2006.01) C07K 16/08 (2006.01) C12P 21/08 (2006.01) C12Q 1/02 (2006.01) C40B 40/08 (2006.01) C40B 50/06 (2006.01) G01N 33/53 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2633601
The present Invention provides novel methods for immortalizing cells that secrete antibodies of one or more specific isotypes. Polyclonal, oligoclonal, and monoclonal populations of cells obtained using the methods of the Invention can be screened on the basis of the functional and/or binding activities of the antibodies they secrete, for example directed to antigens of human or viral origin having medical interest, in cell culture conditions. Using these methods, human B cells that secrete antibodies binding human Cytomegalovirus, Herpes Simplex Virus, or HSP60 protein have been efficiently immortalized with Epstein-Barr virus.
L'invention concerne des procédés permettant d'immortaliser des cellules sécrétant des anticorps d'un ou de plusieurs isotypes spécifiques. Des populations polyclonales, oligoclonales et monoclonales des cellules obtenues au moyen des procédés selon l'invention peuvent être criblées en fonction des activités fonctionnelles et/ou de liaison des anticorps qu'elles sécrétent, par exemple dirigés contre des antigènes d'origine humaine ou virale présentant un intérêt médical, dans des conditions de culture de cellules. L'utilisation de ces procédés permet d'immortaliser, de manière efficace, au moyen du virus d'Epstein-Barrdes des cellules B humaines sécrétant des anticorps se liant au cytomégalovirus humain, au virus de l'herpès simplex humain ou à la protéine HSP60 humaine.
Funaro Ada
Garotta Gianni
Murphy Marianne
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Ribovax Biotechnologies Sa
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