Vectors and methods for expression of mutant proteins

Details Vectors and methods for expression of mutant proteins Vectors and methods for expression of mutant proteins

C - Chemistry, Metallurgy

12

N

C12N 15/77 (2006.01) A61K 47/48 (2006.01) C07K 14/34 (2006.01) C12N 15/81 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2283497

The present invention describes a new shuttle vector permitting the expression of engineered toxin mutants and toxin fusion proteins in Corynebacterium. In addition the invention provides a mutant Pichia pastoris, a method for producing this mutant and a method of expressing engineered toxin mutants and toxin fusion proteins in the mutant form of Pichia pastoris. The invention further provides a mutant Chinese hamster ovary (CHO) cell, a mutant insect cell, a method for producing these mutants and a method of expressing engineered toxin mutants and toxin fusion proteins in the mutant cells.

Nouveau vecteur navette permettant l'expression, dans Corynebacterium, de mutants de toxine et de proteines hybrides de toxine, produits par génie génétique. Cette invention concerne également un Pichia pastoris mutant, un procédé de production de ce mutant, et un procédé permettant l'expression dans la forme mutante de Pichia pastoris, des mutants de toxine et des protéines hybrides de toxine produits par génie génétique. On décrit également une cellule ovarienne de hamster chinois mutant, une cellule d'insecte mutant, un procédé de production de ces mutants et un procédé permettant l'expression dans les cellules de mutants, des mutants de toxine et des protéines hybrides de toxine produits par génie génétique.

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