Microfluidic systems for size based removal of red blood...

G - Physics – 01 – N

Patent

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G01N 1/34 (2006.01) G01N 1/40 (2006.01)

Patent

CA 2529285

The invention features devices and methods for enriching a sample in one or more desired particles. An exemplary use of these devices and methods is for the enrichment of cells, e.g., white blood cells in a blood sample. In general, the methods of the invention employ a device that contains at least one sieve through which particles of a given size, shape, or deformability can pass. Devices of the invention have at least two outlets, and the sieve is placed such that a continuous flow of fluid can pass through the device without passing through the sieve. The devices also include a force generator for directing selected particles through the sieve. Such force generators employ, for example, diffusion, electrophoresis, dielectrophoresis, centrifugal force, or pressure-driven flow.

L'invention concerne des dispositifs et des procédés d'enrichissement d'un échantillon en une ou en plusieurs particules voulues. Une utilisation prise à titre d'exemple de ces dispositifs et procédés vise à l'enrichissement de cellules, par exemple, des globules blancs dans un échantillon de sang. D'une manière générale, les procédés de l'invention utilisent un dispositif contenant au moins un tamis par lequel peuvent passer des particules d'une taille, d'une forme ou d'une déformabilité donnée. Les dispositifs de l'invention comportent au moins deux sorties et le tamis est placé de telle sorte qu'un flux continu de fluide peut passer à travers le dispositif sans passer à travers le tamis. Les dispositifs comprennent également un générateur de forces destiné à diriger des particules sélectionnées à travers le tamis. Lesdits générateurs de forces utilisent, par exemple, la diffusion, l'électrophorèse, la diélectrophorèse, la force centrifuge ou un flux entraîné par pression.

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