Method for immortalizing cells

Details Method for immortalizing cells Method for immortalizing cells

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C07K 14/47 (2006.01) C12N 7/00 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2263790

This invention relates to the introduction of p53 under the control of the metallothionein promoter into primary cells to produce immortalized cell lines. The cells are useful as substrates for viral propagation, as contaminant-free sources for recombinant protein production, for recombinant virus production and as cell substrates to support primary cells and improve virus yield during virus propagation.

L'invention concerne l'introduction de p53 du promoteur de métallothionéine dans des cellules primaires sous le contrôle afin de produire des lignées cellulaires immortalisées. Lesdites cellules peuvent servir en tant que substrats pour la propagation de virus, en tant que sources exemptes de contaminants pour la production de protéines recombinées, pour la production de virus recombinés et en tant que substrats cellulaires destinés à servir de support à des cellules primaires et à améliorer le rendement de virus lors de la propagation de virus.

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