Culture medium and method for inducing differentiation into...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/07 (2010.01) C12N 15/09 (2006.01)

Patent

CA 2753299

Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum- free or contains a low concentration of serum.

L'invention concerne un milieu de culture, un additif et un procédé pour induire la différenciation de cellules souches somatiques de mammifères dans des cellules présentant des propriétés caractéristiques d'adipocytes à efficacité élevée dans des conditions utilisant un milieu de culture sans sérum ou à faible teneur en sérum. Plus précisément, l'invention concerne un milieu de culture permettant d'induire la différenciation de cellules souches somatiques de mammifères dans des adipocytes, qui comprend un milieu de culture de base permettant de cultiver des cellules mammifères, un agent pour induire la différenciation de cellules souches somatiques de mammifères dans des adipocytes, la biotine, un ligand pour un récepteur de la famille d'un gène de différenciation de cellules endothéliales (Edg), la vitamine C et HEPES, et qui est sans sérum ou qui présente une faible teneur en sérum.

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