Polynucleotide sequence of fruit softening associated...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/24 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2726292

The present invention provides isolated polynucleotide sequences encoding .beta.-D-N-acetylhexosaminidase. The present invention further provides DNA construct comprising the polynucleotide sequence coding for .beta.-D-N-acetylhexosaminidase in sense or anti-sense orientation, RNAi construct, recombinant vector comprising the construct and host cells comprising the recombinant vector disclosed in the present invention. The present invention further provides transgenic plant, plant cell, transgenic progeny and seeds expressing the polynucleotide with reduced .beta.-D-N-acetylhexosaminidase protein accumulation, having enhanced fruit shelf life.

L'invention concerne des séquences polynucléotidiques isolées codant pour une ß-D-N-acétylhexosaminidase. L'invention concerne également une construction d'ADN comprenant la séquence polynucléotidique codant pour une ß-D-N-acétylhexosaminidase dans une orientation sens ou antisens, une construction d'ADNi, un vecteur recombinant comprenant la construction et des cellules hôtes comprenant le vecteur recombinant décrit dans l'invention. L'invention concerne enfin une plante transgénique, une cellule végétale, une descendance et des graines transgéniques exprimant le polynucléotique avec une accumulation de protéine de ß-D-N-acétylhexosaminidase limitée et présentant une durée de vie des fruits améliorée.

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