Multi-nucleated cell classification and micronuclei scoring

Details Multi-nucleated cell classification and micronuclei scoring Multi-nucleated cell classification and micronuclei scoring

G - Physics

06

K

G06K 9/00 (2006.01) G06K 9/34 (2006.01) G06K 9/48 (2006.01)

Patent

CA 2746743

Methods and systems for counting nuclei for cells in a cell-containing sample are disclosed. The methods comprise: receiving a raw image of the cell-containing sample; transforming the raw image into a segmented image comprising one or more nuclei clusters. The methods further comprise: for each of the one or more nuclei clusters, obtaining a convex hull of the nuclei cluster; locating any indentations on the nuclei cluster by comparing the nuclei cluster to the convex hull of the nuclei cluster; calculating a first nuclei count based on a tally of the indentations; and assigning the nuclei cluster to a cell among the plurality of cells. The methods further comprise: calculating a second nuclei count for each cell by totaling the first nuclei counts of its constituent nuclei clusters; and presenting a result based on the second nuclei count for at least one of the plurality of cells.

L'invention concerne des procédés et des systèmes permettant de compter les noyaux de cellules dans un échantillon contenant des cellules. Les procédés comprennent la réception d'une image brute de l'échantillon contenant des cellules; la transformation de l'image brute en une image segmentée comprenant un ou plusieurs groupes de noyaux. Les procédés comprennent en outre l'obtention d'une coque convexe pour le ou les groupes de noyaux; la localisation d'indentations dans le groupe de noyaux par la comparaison du groupe de noyaux à la coque convexe du groupe de noyaux; le calcul d'un premier total de noyaux sur la base d'un décompte des indentations; et l'attribution du groupe de noyaux à une cellule choisie parmi la pluralité de cellules. Les procédés comprennent en outre le calcul d'un second total de noyaux pour chaque cellule par l'addition des premiers totaux de noyaux de leurs groupes de noyaux constitutifs; et la présentation d'un résultat basé sur le second total de noyaux pour au moins une cellule de la pluralité de cellules.

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