Method for the evolutionary design of biochemical reaction...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) C12N 1/00 (2006.01) C12N 15/63 (2006.01) C12Q 1/02 (2006.01) C12Q 3/00 (2006.01) G06F 17/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2434968

The present invention relates to methods for achieving an optimal function of a biochemical reaction network. The methods can be performed in silico using a reconstruction of a biochemical reaction network of a cell an iterative optimization procedures. The methods can further include laboratory culturing steps to confirm and possibly expand the determinations made using the in silico methods, and to produce a cultured cell, or population of cells, with optimal functions. The current invention includes computer systems and computer products including computer-readable program code for performing the in silico steps of the invention.

La présente invention concerne des procédés permettant d'obtenir une fonction optimale d'un réseau de réactions biochimiques. On peut réaliser ces procédés <i>in silico</i> en utilisant une reconstruction de réseau de réactions biochimique cellulaires et par des procédures d'optimisation itératives. Ces procédés peuvent aussi comprendre des étapes de culture en laboratoire destinées à confirmer et éventuellement à étendre les déterminations obtenues par ces procédés <i>in silico</i>, et destinées à produire une cellule de culture ou une population de cellules de culture avec des fonctions optimales. Cette invention comprend un système informatique et des composants informatiques, notamment un code de programme lisible par un ordinateur destiné à effectuer les étapes <i>in silico</i> de cette invention.

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