Mixture of at least two fusion proteins, the production...

Details Mixture of at least two fusion proteins, the production... Mixture of at least two fusion proteins, the production...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12Q 1/02 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2511830

The invention relates to a protein mixture containing at least one first fusion protein containing a protein or a protein fragment, an interaction domain, and a protein translocation sequence which causes the fusion protein to be translocated by the cytoplasmatic membrane when expressed in a bacterium in an essentially unfolded state, and at least one second fusion protein containing a protein or a protein fragment, an interaction domain, and a protein translocation sequence which causes the fusion protein to be translocated by the cytoplasmatic membrane when expressed in a bacterium in an essentially folded state. The interaction domain of the first fusion protein can bind to that of the second fusion protein.

La présente invention concerne un mélange protéique contenant: au moins une première protéine de fusion qui comprend une protéine ou à un fragment protéique, un domaine d'interaction et une séquence de translocation protéique qui provoque la translocation de la protéine de fusion au cours de l'expression dans une bactérie dans un état sensiblement non replié à travers la membrane cytoplasmique; et au moins une seconde protéine de fusion qui comprend une protéine ou un fragment protéique, un domaine d'interaction et une séquence de translocation protéique qui provoque la translocation de la protéine de fusion au cours de l'expression dans une bactérie dans un état sensiblement replié à travers la membrane cytoplasmique, le domaine d'interaction de la première protéine de fusion pouvant se lier à celui de la seconde protéine de fusion.

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