Improved method for the prevention of carryover...

Details Improved method for the prevention of carryover... Improved method for the prevention of carryover...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2670300

An improved method of preventing carryover contamination of an amplification reaction involves treating uracil-containing DNA with uracil-N- DNA glycosylase and heating the DNA in the presence of polyamines, such as spermidine, spermine and the like. Alternatively, the after treatment with uracil-N-DNA glycosylase, the reaction is further incubated with an enzyme having AP lyase activity.

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