Protein monitoring the activity of low-molecular weight...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 19/00 (2006.01) A01K 67/033 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C07K 14/82 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) C12Q 1/02 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2419503

A protein monitoring the activity of a low-molecular weight GTP-binding protein which makes it possible to measure the activation of a non-invasive low-molecular weight GTP-binding protein; a gene encoding the above protein; an expression vector containing this gene; transformed cells and a transgenic animal expressing the above-described protein and carrying the above-described expression vector which is useful in measuring the activation of a non- invasive low-molecular weight GTP-binding protein; a method of measuring the activation of a low-molecular weight GTP-binding protein with the use of the above-described protein; and a method of screening a substance controlling the activity of a low-molecular weight GTP-binding protein.

L'invention concerne la surveillance protéique de l'activité d'une protéine de liaison de GTP (guanosine triphosphate) de bas poids moléculaire, permettant de mesurer l'activation d'une protéine de liaison de GTP de bas poids moléculaire et non effractive. L'invention concerne également un gène codant la protéine ci-dessus, un vecteur d'expression contenant ce gène, des cellules transformées ainsi qu'un animal transgénique exprimant la protéine ci-dessus décrite et portant le vecteur d'expression ci-dessus décrit, lequel est utile pour mesurer l'activation d'une protéine de liaison de GTP de bas poids moléculaire et non effractive. L'invention concerne encore un procédé de mesure de l'activation de cette protéine de liaison, à l'aide de la protéine ci-dessus décrite, ainsi qu'un procédé de criblage d'une substance régulant l'activité d'une protéine de liaison de GTP, de bas poids moléculaire.

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