Antigen detection method involving an oligonucleotide...

Details Antigen detection method involving an oligonucleotide... Antigen detection method involving an oligonucleotide...

G - Physics

01

N

G01N 33/558 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2672354

The present invention refers to a rapid immunochromatographic test device for antigen detection, comprising a first and a second conjugate releasing pad, wherein the first conjugate pad comprises a gold conjugated protein linked first oligonucleotide and a gold conjugated antigen specific antibody, and the second conjugate pad comprises a gold conjugated protein linked second oligonucleotide, which second oligonucleotide is complementary to the first oligonucleotide. The present invention further refers to a use of such test device for antigen detection in urine or saliva, e.g. human choriogonadotropin (hCG) in urine. Embodiments of the test device are a test strip and a detection cup. The present invention also refers to a method for manufacturing such test device.

L'invention concerne un dispositif d'essai immunochromatographique rapide pour la détection d'antigènes, lequel dispositif comprend un premier et un second tampon de libération de conjugué, le premier tampon de conjugué comprenant un premier oligonucléotide lié à une protéine conjuguée à de l'or et un anticorps spécifique d'antigène conjugué à de l'or, et le second tampon de conjugué comprenant un second oligonucléotide lié à une protéine conjuguée à de l'or, lequel second oligonucléotide est complémentaire du premier oligonucléotide. L'invention se rapporte en outre à l'utilisation du dispositif d'essai pour la détection d'antigènes dans l'urine ou la salive, p.ex. la choriogonadotropine humaine (hCG) dans l'urine. Dans des modes de réalisation, le dispositif d'essai est constitué d'une bandelette réactive et d'un récipient de détection. L'invention concerne également un procédé de fabrication du dispositif d'essai précité.

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