Production of variant nisin

Details Production of variant nisin Production of variant nisin

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) C07K 14/315 (2006.01) C12N 1/21 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2205204

A cell which does not contain a natural nisA gene but expresses a nisin comprising a variant nisA gene wherein the variant nisA gene has the same relationship as the natural nisA gene to a gene cluster containing the natural nisA gene and the genes for nisin modification, secretion and immunity. Preferably the natural, chromosomal nisA gene is absent and the cell comprises a variant nisA gene at the chromosomal location of the said natural nisA gene. Methods of making the cells, and processes for producing nisin, in particular variant nisin, are described.

Une cellule qui ne contient pas le gène nisA naturel mais exprime une nisine comprenant une variante du gène nisA, lequel possède la même relation que le gène nisA naturel par rapport à un groupe de gènes contenant le gène nisA naturel ainsi que les gènes de modification, de sécrétion et d'immunité de la nisine. De préférence, le gène nisA chromosomique est absent et la cellule comprend une variante du gène nisA à l'emplacement chromosomique dudit gène nisA naturel nisA. L'invention concerne également des procédés de production de ces cellules ainsi que des procédés de production de nisine, notamment d'une nisine à variante particulière.

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